A Single Nucleotide Mutation in Adenylate Cyclase Affects Vegetative Growth, Sclerotial Formation and Virulence of Botrytis cinerea.

ABSTRACT: Botrytis cinerea is a pathogenic fungus that causes gray mold disease in a broad range of crops. The high intraspecific variability of B. cinerea makes control of this fungus very difficult. Here, we isolated a variant B05.10^M strain from wild-type B05.10. The B05.10^M strain showed serious defects in mycelial growth, spore and sclerotia production, and virulence. Using whole-genome resequencing and site-directed mutagenesis, a single nucleotide mutation in the adenylate cyclase (BAC) gene that results in an amino acid residue (from serine to proline, S1407P) was shown to be the cause of various defects in the B05.10^M strain. When we further investigated the effect of S1407 on BAC function, the S1407P mutation in bac showed decreased acc

SUBMITTER: Chen X 

PROVIDER: S-EPMC7215688 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature