Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum.

ABSTRACT: BACKGROUND:Acetoin, especially the optically pure (3S)- or (3R)-enantiomer, is a high-value-added bio-based platform chemical and important potential pharmaceutical intermediate. Over the past decades, intense efforts have been devoted to the production of acetoin through green biotechniques. However, efficient and economical methods for the production of optically pure acetoin enantiomers are rarely reported. Previously, we systematically engineered the GRAS microorganism Corynebacterium glutamicum to efficiently produce (3R)-acetoin from glucose. Nevertheless, its yield and average productivity were still unsatisfactory for industrial bioprocesses. RESULTS:In this study, cellular carbon fluxes in the acetoin producer CGR6 were further redirected toward acetoin synthesis using several met

SUBMITTER: Lu L 

PROVIDER: S-EPMC7216327 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature