Transcriptional analysis of the multiple Sry genes and developmental program at the onset of testis differentiation in the rat.

ABSTRACT:

Background

The commonly used laboratory rat, Rattus norvegicus, is unique in having multiple Sry gene copies found on the Y chromosome, with different copies encoding amino acid variations that influence the resulting protein function. It is not clear which Sry genes are expressed at the onset of testis differentiation or how their expression correlates with that of other genes in testis-determination pathways.

Methods

Here, two independent E11-E14 developmental RNAseq datasets show that multiple Sry genes are expressed at E12-E13.

Results

The identified copies expressed during testis initiation include Sry4A, Sry1, and Sry3C, which are conserved in every strain of Rattus norvegicus with genomes sequenced to date.

Conclusions

This work represents a first step i

SUBMITTER: Prokop JW 

PROVIDER: S-EPMC7216353 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature