Activated CAMKKβ-AMPK signaling promotes autophagy in a spheroid model of ovarian tumour metastasis.

ABSTRACT:

Background

A hallmark of epithelial ovarian cancer (EOC) metastasis is the process of spheroid formation, whereby tumour cells aggregate into 3D structures while in suspension in the peritoneal cavity. EOC spheroids are subjected to bioenergetic stress, thereby activating AMP-activated protein kinase (AMPK) signaling to enter a metabolically quiescent state, which can facilitate cell survival under nutrient-limiting conditions. Independently, we have also demonstrated that EOC spheroids induce autophagy, a process that degrades and recycles intracellular components to restore energy and metabolites. Herein, we sought to examine whether AMPK controls autophagy induction as a cell survival mechanism in EOC spheroids.

Results

We observed a co-ordinate increase in phosphorylated

SUBMITTER: Laski J 

PROVIDER: S-EPMC7216359 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature