High-resolution profile of transcriptomes reveals a role of alternative splicing for modulating response to nitrogen in maize.

ABSTRACT:

Background

The fluctuation of nitrogen (N) contents profoundly affects the root growth and architecture in maize by altering the expression of thousands of genes. The differentially expressed genes (DEGs) in response to N have been extensively reported. However, information about the effects of N variation on the alternative splicing in genes is limited.

Results

To reveal the effects of N on the transcriptome comprehensively, we studied the N-starved roots of B73 in response to nitrate treatment, using a combination of short-read sequencing (RNA-seq) and long-read sequencing (PacBio-sequencing) techniques. Samples were collected before and 30 min after nitrate supply. RNA-seq analysis revealed that the DEGs in response to N treatment were mainly associated with N metabolism

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7216474 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature