Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation.

ABSTRACT:

Background

Gene editing using the CRISPR/Cas9 system has become a routinely applied method in several plant species. The most convenient gene delivery system is Agrobacterium-mediated gene transfer with antibiotic selection and stable genomic integration of transgenes, including Cas9. For elimination of transgenes in the segregating progeny, selfing is applied in many plant species. This approach, however, cannot be widely employed in potato because most of the commercial potato cultivars are self-incompatible.

Results

In this study, the efficiency of a transient Cas9 expression system with positive/negative selection based on codA-nptII fusion was tested. The PHYTOENE DESATURASE (PDS) gene involved in carotenoid biosynthesis was targeted. A new vector designated PROGED::gPD

SUBMITTER: Banfalvi Z 

PROVIDER: S-EPMC7216596 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature