In vitro and in vivo recombination of heterologous modules for improving biosynthesis of astaxanthin in yeast.

ABSTRACT:

Background

Astaxanthin is a kind of tetraterpene and has strong antioxygenic property. The biosynthesis of astaxanthin in engineered microbial chassis has greater potential than its chemical synthesis and extraction from natural producers in an environmental-friendly way. However, the cost-offsetting production of astaxanthin in engineered microbes is still constrained by the poor efficiency of astaxanthin synthesis pathway as a heterologous pathway.

Results

To address the bottleneck of limited production of astaxanthin in microbes, we developed in vitro and in vivo recombination methods respectively in engineered yeast chassis to optimize the combination of heterologous β-carotene ketolase (crtW) and hydroxylase (crtZ) modules that were selected from different species. As a

SUBMITTER: Qi DD 

PROVIDER: S-EPMC7216642 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature