High-throughput three-dimensional visualization of root system architecture of rice using X-ray computed tomography.

ABSTRACT:

Background

X-ray computed tomography (CT) allows us to visualize root system architecture (RSA) beneath the soil, non-destructively and in a three-dimensional (3-D) form. However, CT scanning, reconstruction processes, and root isolation from X-ray CT volumes, take considerable time. For genetic analyses, such as quantitative trait locus mapping, which require a large population size, a high-throughput RSA visualization method is required.

Results

We have developed a high-throughput process flow for the 3-D visualization of rice (Oryza sativa) RSA (consisting of radicle and crown roots), using X-ray CT. The process flow includes use of a uniform particle size, calcined clay to reduce the possibility of visualizing non-root segments, use of a higher tube voltage and cu

SUBMITTER: Teramoto S 

PROVIDER: S-EPMC7216661 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature