High-Resolution Three-Dimensional Imaging of Individual Astrocytes Using Confocal Microscopy.

ABSTRACT: Astrocytes play numerous vital roles in the central nervous system. Accordingly, it is of merit to identify structural and functional properties of astrocytes in both health and disease. The majority of studies examining the morphology of astrocytes have employed immunoassays for markers such as glial fibrillary acidic protein, which are insufficient to encapsulate the considerable structural complexity of these cells. Herein, we describe a method utilizing a commercially available and validated, genetically encoded membrane-associated fluorescent marker of astrocytes, AAV5-GfaABC1D-Lck-GFP. This tool and approach allow for visualization of a single isolated astrocyte in its entirety, including fine peripheral processes. Astrocytes are imaged using confocal microscopy and reconstructed in

SUBMITTER: Testen A 

PROVIDER: S-EPMC7216730 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature