Sequencing smart: De novo sequencing and assembly approaches for a non-model mammal.

ABSTRACT:

Background

Whilst much sequencing effort has focused on key mammalian model organisms such as mouse and human, little is known about the relationship between genome sequencing techniques for non-model mammals and genome assembly quality. This is especially relevant to non-model mammals, where the samples to be sequenced are often degraded and of low quality. A key aspect when planning a genome project is the choice of sequencing data to generate. This decision is driven by several factors, including the biological questions being asked, the quality of DNA available, and the availability of funds. Cutting-edge sequencing technologies now make it possible to achieve highly contiguous, chromosome-level genome assemblies, but rely on high-quality high molecular weight DNA. However, fun

SUBMITTER: Etherington GJ 

PROVIDER: S-EPMC7216774 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature