Human airway construct model is suitable for studying transcriptome changes associated with indoor air particulate matter toxicity.

ABSTRACT: In vitro models mimicking the human respiratory system are essential when investigating the toxicological effects of inhaled indoor air particulate matter (PM). We present a pulmonary cell culture model for studying indoor air PM toxicity. We exposed normal human bronchial epithelial cells, grown on semi-permeable cell culture membranes, to four doses of indoor air PM in the air-liquid interface. We analyzed the chemokine interleukin-8 concentration from the cell culture medium, protein concentration from the apical wash, measured tissue electrical resistance, and imaged airway constructs using light and transmission electron microscopy. We sequenced RNA using a targeted RNA toxicology panel for 386 genes associated with toxicological responses. PM was collected from a non-complaint reside

SUBMITTER: Nordberg ME 

PROVIDER: S-EPMC7217003 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature