KIAA1429 promotes osteosarcoma progression by promoting stem cell properties and is regulated by miR-143-3p.

ABSTRACT: Background: Osteosarcoma (OS) is the most common primary bone malignancy, it has a dismal prognosis and mainly affects the children and adolescents. Previous reports have demonstrated that aberrantly expressed KIAA1429 plays crucial roles in the carcinogenesis of several cancers, but its expression status and functional role in the progression of OS have not previously been investigated.Methods: Immunohistochemistry (IHC) and western blotting were conducted to determine KIAA1429 expression status in OS. The relationship between KIAA1429 expression and OS prognosis was analyzed based on public database and tissue microarray (TMA). Cell proliferation ability was evaluated by CCK8, EdU and colony formation assays, and Transwell and wound healing potential were also assessed i

SUBMITTER: Han Q 

PROVIDER: S-EPMC7217356 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature