Local emergence in Amazonia of <i>Plasmodium falciparum k13</i> C580Y mutants associated with <i>in vitro</i> artemisinin resistance.

ABSTRACT: Antimalarial drug resistance has historically arisen through convergent de novo mutations in Plasmodium falciparum parasite populations in Southeast Asia and South America. For the past decade in Southeast Asia, artemisinins, the core component of first-line antimalarial therapies, have experienced delayed parasite clearance associated with several pfk13 mutations, primarily C580Y. We report that mutant pfk13 has emerged independently in Guyana, with genome analysis indicating an evolutionary origin distinct from Southeast Asia. Pfk13 C580Y parasites were observed in 1.6% (14/854) of samples collected in Guyana in 2016-2017. Introducing pfk13 C580Y or R539T mutations by gene editing into local parasites conferred high levels of in vitro arte

SUBMITTER: Mathieu LC 

PROVIDER: S-EPMC7217694 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature