Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells.

ABSTRACT:

Purpose

Amniotic membrane stem cells have a high capacity of proliferation, cell expansion, and plasticity, as well as immunomodulatory properties that contribute to maternal-fetal tolerance. Owing to the lack of research on human amniotic membrane at different gestational stages, the canine model is considered ideal because of its genetic and physiological similarities. We aimed to characterize the canine amniotic membrane (CAM) cell lineage in different gestational stages and evaluate the expression of immunomodulatory genes.

Materials and methods

Twenty CAMs from early (20-30 days) (n=7), mid- (31-45 days) (n=7), and late gestation (46-63 days) (n=6) stages were studied. The cell features were assessed by cell viability tests, growth curve, colony-forming units, in vitro

SUBMITTER: de Oliveira Pinheiro A 

PROVIDER: S-EPMC7217707 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature