Toll-like receptor signaling in thymic epithelium controls monocyte-derived dendritic cell recruitment and Treg generation.

ABSTRACT: The development of thymic regulatory T cells (Treg) is mediated by Aire-regulated self-antigen presentation on medullary thymic epithelial cells (mTECs) and dendritic cells (DCs), but the cooperation between these cells is still poorly understood. Here we show that signaling through Toll-like receptors (TLR) expressed on mTECs regulates the production of specific chemokines and other genes associated with post-Aire mTEC development. Using single-cell RNA-sequencing, we identify a new thymic CD14⁺Sirpα⁺ population of monocyte-derived dendritic cells (CD14⁺moDC) that are enriched in the thymic medulla and effectively acquire mTEC-derived antigens in response to the above chemokines. Consistently, the cellularity of CD14⁺moDC is diminished in mice w

SUBMITTER: Voboril M 

PROVIDER: S-EPMC7217920 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature