Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1.

ABSTRACT: Emerging evidence identifies major contributions of platelets to inflammatory amplification in dengue, but the mechanisms of infection-driven platelet activation are not completely understood. Dengue virus nonstructural protein-1 (DENV NS1) is a viral protein secreted by infected cells with recognized roles in dengue pathogenesis, but it remains unknown whether NS1 contributes to the inflammatory phenotype of infected platelets. This study shows that recombinant DENV NS1 activated platelets toward an inflammatory phenotype that partially reproduced DENV infection. NS1 stimulation induced translocation of α-granules and release of stored factors, but not of newly synthesized interleukin-1β (IL-1β). Even though both NS1 and DENV were able to induce pro-IL-1β synthesis, only DENV infection tr

SUBMITTER: Quirino-Teixeira AC 

PROVIDER: S-EPMC7218431 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature