A PopZ-linked apical recruitment assay for studying protein-protein interactions in the bacterial cell envelope.

ABSTRACT: Most bacteria are surrounded by a complex cell envelope. As with many biological processes, studies of envelope assembly have benefited from cell-based assays for detecting protein-protein interactions. These assays use simple readouts and lack a protein purification requirement, making them ideal for early stage investigations. The most widely used two-hybrid interaction assay for proteins involved in envelope biogenesis is based on the reconstitution of adenylate cyclase activity from a split enzyme. Because adenylate cyclase is only functional in the cytoplasm, both protein fusions used in the assay must have a terminus located in this compartment. However, many envelope assembly factors are wholly extracytoplasmic. Detecting interactions involving such proteins using two-hybrid systems

SUBMITTER: Lim HC 

PROVIDER: S-EPMC7218919 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature