Development of an Aptamer Based Luminescent Optical Fiber Sensor for the Continuous Monitoring of Hg2+ in Aqueous Media.

ABSTRACT: A fluorescent optical fiber sensor for the detection of mercury (Hg2+) ions in aqueous solutions is presented in this work. The sensor was based on a fluorophore-labeled thymine (T)-rich oligodeoxyribonucleotide (ON) sequence that was directly immobilized onto the tip of a tapered optical fiber. In the presence of mercury ions, the formation of T-Hg2+-T mismatches quenches the fluorescence emission by the labeled fluorophore, which enables the measurement of Hg2+ ions in aqueous solutions. Thus, in contrast to commonly designed sensors, neither a fluorescence quencher nor a complementary ON sequence is required. The sensor presented a response time of 24.8 seconds toward 5 × 10-12 M Hg2+. It also showed both good reversibility (higher than the 95.8%) and selectivity: the I0/I variation was

SUBMITTER: De Acha N 

PROVIDER: S-EPMC7219322 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature