Differential Expression of IgM and IgD Discriminates Two Subpopulations of Human Circulating IgM⁺IgD⁺CD27⁺ B Cells That Differ Phenotypically, Functionally, and Genetically.

ABSTRACT: The origin and function of blood IgM⁺IgD⁺CD27⁺ B cells is controversial, and they are considered a heterogeneous population. Previous staining of circulating B cells of healthy donors with rotavirus fluorescent virus-like particles allowed us to differentiate two subsets of IgM⁺IgD⁺CD27⁺: IgM^hi and IgM^lo B cells. Here, we confirmed this finding and compared the phenotype, transcriptome, in vitro function, and Ig gene repertoire of these two subsets. Eleven markers phenotypically discriminated both subsets (CD1c, CD69, IL21R, CD27, MTG, CD45RB, CD5, CD184, CD23, BAFFR, and CD38) with the IgM^hi phenotypically resembling previously reported marginal zone B cells and the IgM^lo r

SUBMITTER: Bautista D 

PROVIDER: S-EPMC7219516 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature