Versatile Tool for Droplet Generation in Standard Reaction Tubes by Centrifugal Step Emulsification.

ABSTRACT: We present a versatile tool for the generation of monodisperse water-in-fluorinated-oil droplets in standard reaction tubes by centrifugal step emulsification. The microfluidic cartridge is designed as an insert into a standard 2 mL reaction tube and can be processed in standard laboratory centrifuges. It allows for droplet generation and subsequent transfer for any downstream analysis or further use, does not need any specialized device, and manufacturing is simple because it consists of two parts only: A structured substrate and a sealing foil. The design of the structured substrate is compatible to injection molding to allow manufacturing at large scale. Droplets are generated in fluorinated oil and collected in the reaction tube for subsequent analysis. For sample sizes up to 100 µL wi

SUBMITTER: Schulz M 

PROVIDER: S-EPMC7221521 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature