Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy.

ABSTRACT: The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study. In this research, pH-sensitive carbon dots (CDs) were employed for intracellular pH sensing with fluorescence lifetime imaging microscopy (FLIM) for the first time. FLIM is a highly sensitive method that is used to detect a microenvironment and it can overcome the limitations of biosensing methods based on fluorescence intensity. The different groups on the CDs surfaces changing with pH environments led to different fluorescence lifetime values. The CDs aqueous solution had a gradual change from 1.6 ns to 3.7 ns in the fluorescence lifetime with a pH range of 2.6-8.6. Similar fluo

SUBMITTER: Huang M 

PROVIDER: S-EPMC7221822 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature