N⁶-methyladenosine demethylases Alkbh5/Fto regulate cerebral ischemia-reperfusion injury.

ABSTRACT:

Background

Although N⁶-methyladenosine (m⁶A) plays a very important role in different biological processes, its function in the brain has not been fully explored. Thus, we investigated the roles of the RNA demethylases Alkbh5/Fto in cerebral ischemia-reperfusion injury.

Methods

We used a rat model and primary neuronal cell culture to study the role of m⁶A and Alkbh5/Fto in the cerebral cortex ischemic penumbra after cerebral ischemia-reperfusion injury. We used Alkbh5-shRNA and Lv-Fto (in vitro) to regulate the expression of Alkbh5/Fto to study their regulation of m⁶A in the cerebral cortex and to study brain function after ischemia-reperfusion injury.

Results

We found that RNA m⁶A levels increased consecut

SUBMITTER: Xu K 

PROVIDER: S-EPMC7222229 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature