Divergent domains of 28S ribosomal RNA gene: DNA barcodes for molecular classification and identification of mites.

ABSTRACT:

Background

The morphological and molecular identification of mites is challenging due to the large number of species, the microscopic size of the organisms, diverse phenotypes of the same species, similar morphology of different species and a shortage of molecular data.

Methods

Nine medically important mite species belonging to six families, i.e. Demodex folliculorum, D. brevis, D. canis, D. caprae, Sarcoptes scabiei canis, Psoroptes cuniculi, Dermatophagoides farinae, Cheyletus malaccensis and Ornithonyssus bacoti, were collected and subjected to DNA barcoding. Sequences of cox1, 16S and 12S mtDNA, as well as ITS, 18S and 28S rDNA from mites were retrieved from GenBank and used as candidate genes. Sequence alignment and analysis identified 28S rDNA as the suitable target ge

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7222323 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature