Discovery of thermophilic Bacillales using reduced-representation genotyping for identification.

ABSTRACT:

Background

This study demonstrates the use of reduced-representation genotyping to provide preliminary identifications for thermophilic bacterial isolates. The approach combines restriction enzyme digestion and PCR with next-generation sequencing to provide thousands of short-read sequences from across the bacterial genomes. Isolates were obtained from compost, hot water systems, and artesian bores of the Great Artesian Basin. Genomic DNA was double-digested with two combinations of restriction enzymes followed by PCR amplification, using a commercial provider of DArTseq™, Diversity Arrays Technology Pty Ltd. (Canberra, Australia). The resulting fragments which formed a reduced-representation of approximately 2.3% of the genome were sequenced. The sequence tags obtained were aligne

SUBMITTER: Talamantes-Becerra B 

PROVIDER: S-EPMC7222431 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature