Clinical application and evaluation of metagenomic next-generation sequencing in suspected adult central nervous system infection.

ABSTRACT:

Background

Accurate etiology diagnosis is crucial for central nervous system infections (CNS infections). The diagnostic value of metagenomic next-generation sequencing (mNGS), an emerging powerful platform, remains to be studied in CNS infections.

Methods

We conducted a single-center prospective cohort study to compare mNGS with conventional methods including culture, smear and etc. 248 suspected CNS infectious patients were enrolled and clinical data were recorded.

Results

mNGS reported a 90.00% (9/10) sensitivity in culture-positive patients without empirical treatment and 66.67% (6/9) in empirically-treated patients. Detected an extra of 48 bacteria and fungi in culture-negative patients, mNGS provided a higher detection rate compared to culture in patients with (

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7222471 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature