Automated image analysis system for studying cardiotoxicity in human pluripotent stem cell-Derived cardiomyocytes.

ABSTRACT: BACKGROUND:Cardiotoxicity, characterized by severe cardiac dysfunction, is a major problem in patients treated with different classes of anticancer drugs. Development of predictable human-based models and assays for drug screening are crucial for preventing potential drug-induced adverse effects. Current animal in vivo models and cell lines are not always adequate to represent human biology. Alternatively, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) show great potential for disease modelling and drug-induced toxicity screenings. Fully automated high-throughput screening of drug toxicity on hiPSC-CMs by fluorescence image analysis is, however, very challenging, due to clustered cell growth patterns and strong intracellular and intercellular variation in the expres

SUBMITTER: Cao L 

PROVIDER: S-EPMC7222481 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature