Isolation and characterisation of nasoseptal cartilage stem/progenitor cells and their role in the chondrogenic niche.

ABSTRACT:

Background

Since cartilage-derived stem/progenitor cells (CSPCs) were first identified in articular cartilage using differential adhesion to fibronectin, their self-renewal capacity and niche-specific lineage preference for chondrogenesis have propelled their application for cartilage tissue engineering. In many adult tissues, stem/progenitor cells are recognised to be involved in tissue homeostasis. However, the role of nasoseptal CSPCs has not yet been elucidated. Our aim was to isolate and characterise nasoseptal CSPCs alongside nasoseptal chondrocyte populations and determine chondrogenic capacity.

Methods

Here, we isolated nasoseptal CSPCs using differential adhesion to fibronectin and assessed their colony forming efficiency, proliferation kinetics, karyotype and trili

SUBMITTER: Jessop ZM 

PROVIDER: S-EPMC7222513 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature