Dracocephalum moldavica L. Extracts Protect H9c2 Cardiomyocytes against H₂O₂-Induced Apoptosis and Oxidative Stress.

ABSTRACT:

Materials and methods

The petroleum ether (petrol), dichloromethane (CH₂Cl₂), ethyl acetate (EtOAc), and n-butyl alcohol (n-BuOH) fractions were isolated from alcohol extracts of D. moldavica L. Total phenolic and flavonoid contents and in vitro antioxidant activities of different fractions were evaluated. H9c2 cells were then treated with D. moldavica L. extracts before challenging with H₂O₂. Cell viability was determined by colorimetric assay, and ELISA was used to measure the levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD). Apoptosis levels and mitochondrial membrane potential were measured by flow cytometry. The expressions of cell apoptosis regulatory pro

SUBMITTER: Jin M 

PROVIDER: S-EPMC7222556 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature