Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors.

ABSTRACT:

Key message

This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yiel

SUBMITTER: Poborilova Z 

PROVIDER: S-EPMC7223956 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature