Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury.

ABSTRACT: Kidney fibroblasts play a crucial role in dictating tubular cell fate and the outcome of acute kidney injury (AKI). The underlying mechanisms remain to be determined. Here, we found that mTOR signaling was activated in fibroblasts from mouse kidneys with ischemia/reperfusion injury (IRI). Ablation of fibroblast Rheb or Rictor promoted, while ablation of fibroblast Tsc1 protected against tubular cell death and IRI in mice. In tubular cells cultured with conditioned media (CM) from Rheb^-/- or Rictor^-/- fibroblasts, less hepatocyte growth factor (HGF) receptor c-met signaling activation or staurosporine-induced cell apoptosis was observed. While CM from Tsc1^-/- fibroblasts promoted tubular cell c-met signaling activation and inhibited staurosporine-induced cel

SUBMITTER: Gui Y 

PROVIDER: S-EPMC7224397 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature