Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells.

ABSTRACT: Previous studies with various Src family kinase biosensors showed that the nuclear kinase activities are much suppressed compared to those in the cytosol, suggesting that these kinases are regulated differently in the nucleus and in the cytosol. In this study, using Fyn as an example, we first engineered a Fyn biosensor with a light-inducible nuclear localization signal to demonstrate that the Fyn kinase activity is significantly lower in the nucleus than in the cytosol. To understand how different equilibrium states between Fyn and the corresponding phosphatases are maintained in the cytosol and nucleus, we further engineered a Fyn kinase domain with light-inducible nuclear localization signal. The results revealed that the Fyn kinase can be actively transported into the nucleus upon ligh

SUBMITTER: Huang Z 

PROVIDER: S-EPMC7225052 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature