Ontology highlight
ABSTRACT:
SUBMITTER: Frame NM
PROVIDER: S-EPMC7225066 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Frame Nicholas M NM Kumanan Meera M Wales Thomas E TE Bandara Asanga A Fändrich Marcus M Straub John E JE Engen John R JR Gursky Olga O
Journal of molecular biology 20200206 7
Serum amyloid A (SAA) is a plasma protein that transports lipids during inflammation. To explore SAA solution conformations and lipid-binding mechanism, we used hydrogen-deuterium exchange mass spectrometry, lipoprotein reconstitution, amino acid sequence analysis, and molecular dynamics simulations. Solution conformations of lipid-bound and lipid-free mSAA1 at pH~7.4 agreed in details with the crystal structures but also showed important differences. The results revealed that amphipathic α-heli ...[more]