DNA methylation markers that correlate with occult lymph node metastases of non-small cell lung cancer and a preliminary prediction model.

ABSTRACT:

Background

Lymph node (LN) metastasis status is the most important prognostic factor and determines treatment strategy. Methylation alteration is an optimal candidate to trace the signal from early stage tumors due to its early existence, multiple loci and stability in blood. We built a diagnostic tool to screen and identify a set of plasma methylation markers in early stage occult LN metastasis.

Methods

High-throughput targeted methylation sequencing was performed on tissue and matched plasma samples from a cohort of 119 non-small cell lung cancer (NSCLC) patients with a primary lesion of less than 3.0 cm in diameter. The methylation profiles were compared between patients with and without occult LN metastases. We carried out a set of machine-learning analyses on our discov

SUBMITTER: Chen Z 

PROVIDER: S-EPMC7225136 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature