A genetic screen identifies Crat as a regulator of pancreatic beta-cell insulin secretion.

ABSTRACT:

Objectives

Glucose-stimulated insulin secretion is a critical function in the regulation of glucose homeostasis, and its deregulation is associated with the development of type 2 diabetes. Here, we performed a genetic screen using islets isolated from the BXD panel of advanced recombinant inbred (RI) lines of mice to search for novel regulators of insulin production and secretion.

Methods

Pancreatic islets were isolated from 36 RI BXD lines and insulin secretion was measured following exposure to 2.8 or 16.7 mM glucose with or without exendin-4. Islets from the same RI lines were used for RNA extraction and transcript profiling. Quantitative trait loci (QTL) mapping was performed for each secretion condition and combined with transcriptome data to prioritize candidate regula

SUBMITTER: Berdous D 

PROVIDER: S-EPMC7225740 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature