Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales.

ABSTRACT:

Objectives

Two commercially available lateral flow immunochromatographic assays (ICAs) for detection of the major carbapenemases were prospectively assessed for the detection of carbapenemases in Enterobacterales: RESIST-4 O.K.N.V. (Coris BioConcept) and NG-Test CARBA 5 (NG Biotech).

Methods

These two assays were performed prospectively on consecutive Enterobacterales suspected of producing a carbapenemase that were referred to the Belgian National Reference Center for Monitoring Antimicrobial Resistance in Gram-Negative Bacteria between March and June 2018. The intensity of the band corresponding to a carbapenemase for each test was compared using ImageJ software.

Results

Of the 161 isolates tested, a carbapenemase was detected in 91 (60 OXA-48-like, 15 VIM, 9 KPC, 5

SUBMITTER: Bogaerts P 

PROVIDER: S-EPMC7225905 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature