Exogenous NAD⁺ Stimulates MUC2 Expression in LS 174T Goblet Cells via the PLC-Delta/PTGES/PKC-Delta/ERK/CREB Signaling Pathway.

ABSTRACT:

Background

MUC2, a major component of the mucus layer in the intestine, is associated with antimicrobial activity and gut immune system function. Currently, mucin is mainly known for its critical function in defense against toxic molecules and pathogens. In this study, we investigated the stimulatory effects of exogenous nicotinamide adenine dinucleotide (NAD⁺) on the expression of MUC2 in LS 174T goblet cells.

Methods

Genes related to MUC2 synthesis were measured by quantitative real-time PCR (qPCR). To analyze the gene expression profiles of NAD⁺-treated LS 174T goblet cells, RNA sequencing was performed. MUC2 expression in the cells and secreted MUC2 were measured by immunocytochemistry (ICC) and ELISA, respectively.

Results

NAD⁺ si

SUBMITTER: Ma S 

PROVIDER: S-EPMC7226023 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature