Discovering the RNA-Binding Proteome of Plant Leaves with an Improved RNA Interactome Capture Method.

ABSTRACT: RNA-binding proteins (RBPs) play a crucial role in regulating RNA function and fate. However, the full complement of RBPs has only recently begun to be uncovered through proteome-wide approaches such as RNA interactome capture (RIC). RIC has been applied to various cell lines and organisms, including plants, greatly expanding the repertoire of RBPs. However, several technical challenges have limited the efficacy of RIC when applied to plant tissues. Here, we report an improved version of RIC that overcomes the difficulties imposed by leaf tissue. Using this improved RIC method in Arabidopsis leaves, we identified 717 RBPs, generating a deep RNA-binding proteome for leaf tissues. While 75% of these RBPs can be linked to RNA biology, the remaining 25% were previously not known to interact wi

SUBMITTER: Bach-Pages M 

PROVIDER: S-EPMC7226388 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature