The exopolysaccharide-eDNA interaction modulates 3D architecture of Bacillus subtilis biofilm.

ABSTRACT:

Background

Bacterial biofilms are surface-adherent microbial communities in which individual cells are surrounded by a self-produced extracellular matrix of polysaccharides, extracellular DNA (eDNA) and proteins. Interactions among matrix components within biofilms are responsible for creating an adaptable structure during biofilm development. However, it is unclear how the interactions among matrix components contribute to the construction of the three-dimensional (3D) biofilm architecture.

Results

DNase I treatment significantly inhibited Bacillus subtilis biofilm formation in the early phases of biofilm development. Confocal laser scanning microscopy (CLSM) and image analysis revealed that eDNA was cooperative with exopolysaccharide (EPS) in the early stages of B. subtili

SUBMITTER: Peng N 

PROVIDER: S-EPMC7227074 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature