Multiple-centre clinical evaluation of an ultrafast single-tube assay for SARS-CoV-2 RNA.

ABSTRACT:

Objective

To evaluate the performance of an ultrafast single-tube nucleic acid isothermal amplification detection assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA using clinical samples from multiple centres.

Methods

A reverse transcription recombinase-aided amplification (RT-RAA) assay for SARS-CoV-2 was conducted within 15 minutes at 39°C with portable instruments after addition of extracted RNA. The clinical performance of RT-RAA assay was evaluated using 947 clinical samples from five institutions in four regions of China; approved commercial fluorescence quantitative real-time PCR (qRT-PCR) kits were used for parallel detection. The sensitivity and specificity of RT-RAA were compared and analysed.

Results

The RT-RAA test results of 926 s

SUBMITTER: Wang J 

PROVIDER: S-EPMC7227500 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature