Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans.

ABSTRACT: During the aging process, many cells accumulate high levels of damage leading to cellular dysfunction, which underlies many geriatric and pathological conditions. Post-mitotic neurons represent a major cell type affected by aging. Although multiple mammalian models of neuronal aging exist, they are challenging and expensive to establish. The roundworm Caenorhabditis elegans is a powerful model to study neuronal aging, as these animals have short lifespan, an available robust genetic toolbox, and well-cataloged nervous system. The method presented herein allows for seamless isolation of specific cells based on the expression of a transgenic green fluorescent protein (GFP). Transgenic animal lines expressing GFP under distinct, cell type-specific promoters are digested to remove the outer cu

SUBMITTER: Germany EM 

PROVIDER: S-EPMC7227805 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature