An automated data processing and analysis pipeline for transmembrane proteins in detergent solutions.

ABSTRACT: The application of small angle X-ray scattering (SAXS) to the structural characterization of transmembrane proteins (MPs) in detergent solutions has become a routine procedure at synchrotron BioSAXS beamlines around the world. SAXS provides overall parameters and low resolution shapes of solubilized MPs, but is also meaningfully employed in hybrid modeling procedures that combine scattering data with information provided by high-resolution techniques (eg. macromolecular crystallography, nuclear magnetic resonance and cryo-electron microscopy). Structural modeling of MPs from SAXS data is non-trivial, and the necessary computational procedures require further formalization and facilitation. We propose an automated pipeline integrated with the laboratory-information management system ISPyB,

SUBMITTER: Molodenskiy DS 

PROVIDER: S-EPMC7228933 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature