Slow progressors to type 1 diabetes lose islet autoantibodies over time, have few islet antigen-specific CD8⁺ T cells and exhibit a distinct CD95^hi B cell phenotype.

ABSTRACT:

Aims/hypothesis

The aim of this study was to characterise islet autoantibody profiles and immune cell phenotypes in slow progressors to type 1 diabetes.

Methods

Immunological variables were compared across peripheral blood samples obtained from slow progressors to type 1 diabetes, individuals with newly diagnosed or long-standing type 1 diabetes, and healthy individuals. Polychromatic flow cytometry was used to characterise the phenotypic attributes of B and T cells. Islet autoantigen-specific B cells were quantified using an enzyme-linked immunospot (ELISpot) assay and islet autoantigen-specific CD8⁺ T cells were quantified using peptide-HLA class I tetramers. Radioimmunoassays were used to detect islet autoantibodies. Sera were assayed for various chemokines, cy

SUBMITTER: Hanna SJ 

PROVIDER: S-EPMC7228996 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature