Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays.

ABSTRACT: Autofluorescence spectroscopy has emerged in recent years as a powerful tool to report label-free contrast between normal and diseased tissues, both in vivo and ex vivo. We report the development of an instrument employing Single Photon Avalanche Diode (SPAD) arrays to realize real-time multispectral autofluorescence lifetime imaging at a macroscopic scale using handheld single-point fibre optic probes, under bright background conditions. At the detection end, the fluorescence signal is passed through a transmission grating and both spectral and temporal information are encoded in the SPAD array. This configuration allows interrogation in the spectral range of interest in real time. Spatial information is provided by an external camera together with a guiding beam that provides a visual re

SUBMITTER: Lagarto JL 

PROVIDER: S-EPMC7229199 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature