Development of an efficient vitrification method for chondrocyte sheets for clinical application.

ABSTRACT:

Introduction

Regenerative therapy using chondrocyte sheets is effective for osteoarthritis. The clinical application of chondrocyte sheet therapy is expected to be further advanced by the use of a feasible cryopreservation technique. Previously, we developed a chondrocyte sheet vitrification method; however, it was too complex to be used for routine clinical application. Here, we aimed to develop a prototype method for vitrifying chondrocyte sheets for clinical practice.

Methods

We developed a "circulating vitrification bag" as a container to process cell sheets for vitrification in an efficient and sanitary fashion. Moreover, we invented the "vitrification storage box", which is useful for the vitrification of cell sheets, long-term preservation, and transportation. These d

SUBMITTER: Hayashi A 

PROVIDER: S-EPMC7229411 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature