The importance of scoring recognition fitness in spheroid morphological analysis for robust label-free quality evaluation.

ABSTRACT: Because of the growing demand for human cell spheroids as functional cellular components for both drug development and regenerative therapy, the technology to non-invasively evaluate their quality has emerged. Image-based morphology analysis of spheroids enables high-throughput screening of their quality. However, since spheroids are three-dimensional, their images can have poor contrast in their surface area, and therefore the total spheroid recognition by image processing is greatly dependent on human who design the filter-set to fit for their own definition of spheroid outline. As a result, the reproducibility of morphology measurement is critically affected by the performance of filter-set, and its fluctuation can disrupt the subsequent morphology-based analysis. Although the unexpecte

SUBMITTER: Shirai K 

PROVIDER: S-EPMC7229423 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature