Clinical and functional significance of tumor/stromal ATR expression in breast cancer patients.

ABSTRACT: BACKGROUND:Most breast cancer-associated fibroblasts (CAFs) are active and important cancer-promoting cells, with significant impact on patient prognosis. Therefore, we investigated here the role of the protein kinase ATR in breast stromal fibroblasts in the prognosis of locally advanced breast cancer patients. METHODS:We have used immunohistochemistry to assess the level of ATR in breast cancer tissues and their adjacent normal tissues. Immunoblotting as well as quantitative RT-PCR were utilized to show the role of breast cancer cells and IL-6 as well as AUF-1 in downregulating ATR in breast stromal fibroblasts. Engineered human breast tissue model was also used to show that ATR-deficient breast stromal fibroblasts enhance the growth of breast cancer cells. RESULTS:We have shown that the

SUBMITTER: Al-Ansari MM 

PROVIDER: S-EPMC7229635 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature