Global control of bacterial nitrogen and carbon metabolism by a PTS^Ntr-regulated switch.

ABSTRACT: The nitrogen-related phosphotransferase system (PTS^Ntr) of Rhizobium leguminosarum bv. viciae 3841 transfers phosphate from PEP via PtsP and NPr to two output regulators, ManX and PtsN. ManX controls central carbon metabolism via the tricarboxylic acid (TCA) cycle, while PtsN controls nitrogen uptake, exopolysaccharide production, and potassium homeostasis, each of which is critical for cellular adaptation and survival. Cellular nitrogen status modulates phosphorylation when glutamine, an abundant amino acid when nitrogen is available, binds to the GAF sensory domain of PtsP, preventing PtsP phosphorylation and subsequent modification of ManX and PtsN. Under nitrogen-rich, carbon-limiting conditions, unphosphorylated ManX stimulates the TCA cycle and carbon oxidati

SUBMITTER: Sanchez-Canizares C 

PROVIDER: S-EPMC7229655 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature