Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1.

ABSTRACT: One primary objective of synthetic biology is to improve the sustainability of chemical manufacturing. Naturally occurring biological systems can utilize a variety of carbon sources, including waste streams that pose challenges to traditional chemical processing, such as lignin biomass, providing opportunity for remediation and valorization of these materials. Success, however, depends on identifying micro-organisms that are both metabolically versatile and engineerable. Identifying organisms with this combination of traits has been a historic hindrance. Here, we leverage the facile genetics of the metabolically versatile bacterium Acinetobacter baylyi ADP1 to create easy and rapid molecular cloning workflows, including a Cas9-based single-step marker-less and scar-less genomic integration

SUBMITTER: Biggs BW 

PROVIDER: S-EPMC7229861 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature