Ion Transport Basis of Diarrhea in a Mouse Model of Adoptive T Cell Transfer Colitis.

ABSTRACT:

Background

Diarrhea, a major pathological hallmark of inflammatory bowel disease, is characterized by a significant reduction in the expression and function of key intestinal ion transporters. The adoptive naïve CD4⁺ T cell transfer colitis is an immune-based, chronic colitis mouse model which resembles human Crohn's disease. Although mice with T cell transfer colitis demonstrate diarrhea, the ion transporter basis of this phenotype has not been explored.

Aims/methods

In the current studies, we aimed to determine the mRNA and protein levels of the key NaCl transporters DRA and NHE3 along with the mRNA expression of other transporters in the inflamed intestine.

Results

Naïve CD4⁺ T cells, transferred to Rag2 knockout mice, induced severe colonic i

SUBMITTER: Jayawardena D 

PROVIDER: S-EPMC7230007 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature