MinION-Based DNA Barcoding of Preserved and Non-Invasively Collected Wildlife Samples.

ABSTRACT: The ability to sequence a variety of wildlife samples with portable, field-friendly equipment will have significant impacts on wildlife conservation and health applications. However, the only currently available field-friendly DNA sequencer, the MinION by Oxford Nanopore Technologies, has a high error rate compared to standard laboratory-based sequencing platforms and has not been systematically validated for DNA barcoding accuracy for preserved and non-invasively collected tissue samples. We tested whether various wildlife sample types, field-friendly methods, and our clustering-based bioinformatics pipeline, SAIGA, can be used to generate consistent and accurate consensus sequences for species identification. Here, we systematically evaluate variation in cytochrome b sequences amplified

SUBMITTER: Seah A 

PROVIDER: S-EPMC7230362 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature